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KMID : 0545120010110050884
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 5 p.884 ~ p.886
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Reactivity and Stability of Lignostilbene-α, β-Dioxygenase-I in Various pHs, Temperatures, and in Aqueous Organic Solvents
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MAKOTO, NIWA
KAMODA, SHIGEHIRO/SABURI, YOSHIMASA
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Abstract
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The reactivity and stability of purified Lignostilbene-α, βdioxygenase (LSD)-I were examined. Its optimum temperature was 50℃ at pH 8.5, but it was stable only up to 30℃. The activity of LSD-I increased 12-fold by 30% methanol, with increased V_max and lowered K_m＾2. LSD-I was stable in 10% methanol.
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